Absolutely. Since students play cards as fast as they can instead of taking turns, the game will work with more than two players. You'll need a few more cards to do this, so you could either purchase a second set, or just mix in the relevant cards from Level 6 since that level covers the entire range of notes. If you have more than 3-4 students in your group, you can also run a tournament where students play in smaller groups and the winners end up playing each other.

The core game play remains the same, and each deck contains the same number of cards with the same note range as the original set. The main differences are that the Deluxe set: (1) includes level six instead of it being a separate purchase, (2) introduces new wildcards (play as any note value) and "take two" cards (give two cards from your hand to your opponent) that can optionally be added to any level, (3) is printed on real playing card stock so they're more durable and easier to shuffle, (4) has updated artwork with more vibrant colors, and (5) is packaged in a sturdy two piece box with dividers.